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The above results from IR and intrinsic fluorescence spectroscopy strongly suggested that the thermal unfolding of ACYI was a step-wise process
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The sample was thereafter stained using 2% uranyl acetate for 20 s, and the grids were dried overnight
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Samples containing 1-anilinonaphthalene-8-sulfonate (ANS) and Bis-ANS were excited at 290 nm, 370 nm, and 403 nm
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.As described in the Theory section, k^sub ex^ can be derived from the correlation between R^sub ex^ and ΔR^sub ex^ without prior assumption on the timescale of the exchange
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