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A similar difference in emission peak maxima between thermal and GndHCl-induced unfolding was also observed for other proteins (see for example (10,21)) texas student loan forgiveness .
To summarize the above described observations one can state, that GndHCl-induced unfolding reveals for all α-amylases rather uniform emission spectra with large red shifts, whereas thermal unfolded states appear with more divers and in general with much smaller λ^sub max^-values as compared to the former texas student loan forgiveness .
The C-terminal hydrophobic peptide was deleted by insertion of a stop codon to obtain a soluble protein, and the loop 103-136 was replaced by three histidines to facilitate purification texas student loan forgiveness .
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Fluorescence spectra were obtained from samples in quartz cuvettes with 10 mm or 4 mm pathlengths texas student loan forgiveness .
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