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Assisting the reactivation of guanidine hydrochloride-denatured aminoacylase by hydroxypropyl cyclodextrins
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Low temperature induced conformational changes of aminoacylase
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coelicolor has been reported, and the corresponding proteins cloned and expressed (14-16)
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From the changes in the fluorescence intensity, the apparent pK^sub a^ of the titration was 4
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These topics have been thoroughly reviewed elsewhere
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ACKNOWLEDGMENT: The authors thank Dr
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Cross-linking was allowed to proceed for 120 s before quenching by addition of 10 µl of 7% NaBH^sub 4^ in 0
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