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Dissecting the Pretransitional Conformational Changes in Aminoacylase I Thermal DenaturationSu, Jing-TanABSTRACT Aminoacylase I (ACYI) catalyzes the stereospecific hydrolysis of L-acylamino acids and is generally assumed to be involved in the final step of the degradation of intracellular N-acetylated proteins
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Since no high-resolution structure of ACYI is available, all of the Trp fluorophores (Classes A and S, I, II, and III) defined previously (34) were included in the fitting procedure
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Simply stated, chain properties for long chains can be predicted by scaling the corresponding properties for short chains and vice versa
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For all other cases, the move is rejected
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The inhibited enzyme has a denaturation midpoint temperature in D^sub 2^O-solution which is shifted by 10°C from 337
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A minimum estimate of the anisotropy of 1
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Aliquots of the aggregation reaction was withdrawn and assayed to avoid adverse effects of probe binding during the reaction
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2, due to longer rotational correlation time of the aggregates than the fluorescence lifetime of ANS
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RESULTSThermal transitions in lipid-free and in DMPC-bound apoA-2Far-UV CD data of lipid-free apoA-2 were recorded from 5 to 20 µg/ml protein solution in 10 mM Na phosphate, pH 7
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However, because we have compared results across a series of molecules where the RNA sequence (and secondary structure) in the vicinity of the dyes has been held constant, the differences between the characteristics we observe are related primarily to tertiary structural differences between the constructs
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Moreover, even if we could control for all confounders, because quantity is more variable than price, we would likely get more precise parameter estimates from models of price than expenditure
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